PGE2 Enzyme Immunoassay

PGE2 Enzyme Immunoassay

Quality level: 200

Biological source: synthetic

Test: ≥93% (HPLC)

Form: dust

Functional group

carboxylic acid
hydroxyl

Sent in: environment

Storage temperature: −20 ° C

SMILES chain

O [C @@ H] 1CC ([C @ H] (C / C = C \ CCCC (O) = O) [C @ H] 1 / C = C / [C @@ H] (O) CCCCC) = O

InChI

1S / C20H32O5 / c1-2-3-6-9-15 (21) 12-13-17-16 (18 (22) 14-19 (17) 23) 10-7-4-5-8-11- 20 (24) 25 / h4,7,12-13,15-17,19,21,23H, 2-3,5-6,8-11,14H2,1H3, (H, 24,25) / b7- 4-, 13-12 + / t15-, 16 +, 17 +, 19 + / m0 / s1

InChI key

XEYBRNLFEZDVAW-ARSRFYASSA-N

Related categories: Lipids

Description

Packaging

1, 5, 10 mg in glass bottle

Biochemical / Physiological Actions

Most of the biologically active prostaglandins. PGE2 induces cervical ripening and parturition; mediates bradykinin-induced vasodilation; regulates adenylyl cyclase. Tumor cells that overexpress cyclooxygenase 2 show increased invasiveness, angiogenesis, and resistance to apoptosis, which may be due to the expression of angiogenic factors induced by PGE2 and the stabilization of the anti-apoptotic protein, survivin. it is mixed. It inhibits T-cell activation in vitro, suggesting that it is an immunosuppressant. However, in vivo, it appears to affect the expansion of the Th17 subset and differentiation of the Th1 subset of helper T cells, marking it as an immune activator.

About this kit

The Human Prostaglandin E2 ELISA Kit is a competitive enzyme-linked immunosorbent assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples.

Principle of the method

This assay is based on competition between prostaglandin E 2 (PGE2) and an alkaline phosphatase tracer PGE2 for a limited amount of PGE2-specific monoclonal antibody. Due to the competition between PGE2 in the sample and the PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample.

Rigorous validation

Each manufactured batch of this ELISA kit is quality tested based on criteria such as sensitivity, specificity, precision, and batch-to-batch consistency. See the manual for more information on validation.

Destination information

Prostaglandin E2 (PGE2) is a potent lipid mediator produced by arachidonic acid metabolism via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipid deposits by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 different PAGES 1 microsomal enzyme (pages 1), pages 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and environments including inflammation, immune regulation, fever generation, pain perception, gastric muscle protection, fertility, and childbirth, as well as sodium and water retention.

The effects of PGE2 are transduced by 4 subtypes of G protein-coupled receptors called EP1, EP2, EP3, EP4, with Kd values ​​for PGE2 ranging from 110 nM. PGE2 is rapidly metabolized in vivo via the prostaglandin 15 dehydrogenases (15 hydroxy PGDH) pathway to the inactive 13,14 dihydro 15 keto metabolite PGE2. The half-life of PGE2 in the circulatory system is approximately 30 seconds and normal plasma concentrations are 3 to 12 pg/ml.

Leave a Reply

Your email address will not be published.