Product name: Type II collagen (COL2), ELISA kit
Full Product Name: Mouse Type II Collagen ELISA Kit
Product synonym names
COL2 (collagen type II) / chondrocalcin / COL2A1 / SEDC / collagen alpha-1 type II / ANFH / AOM / cartilage collagen / COL11A3 / collagen 2 / collagen chain alpha-1 (II) / collagen II; alpha-1 polypeptide / type 2 collagen
Product gene name: COL2 elisa kit
OMIM: 108300
Species reactivity: Mouse
Samples: Serum, plasma, and other biological fluids.
Test type: Sandwich ELISA, double antibody
Detection range: 62.5-4000 pg / ml
Sensitivity: 37.5 pg / ml
Preparation and storage
Store at 4 degrees C if the kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, lyophilized standard, and concentrated biotinylated detection protein are stored at -20 degrees C. Other components at 2-8 degrees C). Stable for 12 months (if the entire kit is stored at -20 degrees C).
ISO certification
Manufactured in an ISO 13485: 2003 and EN ISO 13485: 2012 Certified Laboratory.
Product note
Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Other notes
Occasionally, small volumes of the COL2 ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Search terms for purchasing COL2
MBS763083 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing the presence of collagen type II (COL2) ELISA kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing COL2.
The analytical biochemical ELISA technique of the MBS763083 kit is based on COL2 antibody-COL2 antigen interactions (immunoadsorption) and an HRP colorimetric detection system to detect COL2 antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant COL2. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
Related Product Information for COL2 ELISA kit
Test principle || This kit was based on sandwich enzyme-linked immunosorbent assay technology. It was precoated with mouse anti-COL2 antibody on 96-well plates. And biotin-conjugated anti-mouse COL2 antibody was used as detection antibodies. Standards, test samples, and biotin-conjugated detection antibodies were subsequently added to the wells and washed with wash buffer.
HRP streptavidin was added and unbound conjugates were washed with wash buffer. TMB substrates were used to visualize the HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue product that changed to yellow after adding an acid stop solution. The density of yellow is proportional to the amount of mouse COL2 sample captured on the plate. Read the D.O. absorbance at 450 nm in a microplate reader, and then the mouse COL2 concentration can be calculated.
